6/05/2018

ANALYSIS OF MOLECULAR BIOLOGY, VIRAL GENETICS AND APPLIED BIOLOGY (UNIT - 3)



ANALYSIS OF MOLECULAR BIOLOGY, VIRAL GENETICS AND APPLIED BIOLOGY

Dr. VIPUL VAIBHAV  (C.C.M.B. HYDERABAD)





At Grassroots, we take the initiative and the responsibility to provide support and guidance to our students beyond classroom teaching.

This analysis deals with the integrated domains of Molecular Biology, Viral Genetics and Genomics. Areas of applied biology including Transgenics and gene therapy etc are also included here.

These areas are highly interesting, being some of the frontier areas of research today...... 
Applied biology areas such as Transgenics, Gene therapy, Marker Assisted Selection (MAS) are already making an impact upon our daily lives. This is possible through application of bio-molecular and Recombinant DNA techniques and genomics. Indeed this does not end here….applying a technique to produce a product (say a GMO) may sound very glamorous and worthy of study. However in order to develop and apply a technology, requires an in-depth understanding of  the basic phenomenon…….in this case, Molecular Biology.  

 Year
Section B
Section C
Jun-211
6
8
Dec-11
6
5
Jun-12
4
8
Dec-12
7
12
Jun-13
8
12
Dec-13
7
8
Jun-14
7
8
Dec-14
5
9
Jun-15
7
10
Dec-15
5
9
Jun-16
5
8
Dec-16
6
11
Jun-17
8
9
Dec-17
6
8
During classroom teaching, we devote 70-80hrs to these domains, supported by extensive illustrations and problem solving. As must be clear from above, students must develop a good base in the basic concepts of molecular biology to understand concepts of Genetic Engineering and applied biology.  

There are no short cuts….

As we analyse the trends of the previous CSIR paper, it is clear that 5-8 questions in part B and 8-12 in part C are from these areas (see table). 

We also performed topic wise analysis related to these domains.

  • It is clear that regulation of gene expression is a key area of focus in these papers. Regulation at the level of transcription, translation, and post-translational modifications are very important.

  • Operons are a frequent focus, specifically the Lac and Trp operon…and in great detail. This does not mean you can skip the other operon systems.

  • In eukaryotic regulation, the role of Regulatory RNAs such as siRNA and miRNA, RNA Interference are important. Questions on regulation of gene expression by DNA methylation and chromatin re-modelling are frequently asked.

  • All these are concept based questions.   Students enjoy focusing on these areas……..….but here comes the rider….regulation  is  difficult to understand without understanding of the basic fundamentals of molecular biology.

  • This is reflected in the fact, that at least twice as many questions  are asked from the fundamentals such as chromatin organization, Gene and genome organization, replication, DNA repair, basic transcription, RNA processing  and Translation. Question from telomeres and its significance have been asked.  

TOPICWISE ANALYSIS OF QUESTIONS

Topics
Jun-11
Dec-11
Jun-12
Dec-12
Jun-13
Dec-13
Jun-14
Dec-14
Jun-15
Dec-15
Jun-16
Dec-16
Jun-17
Dec-17
DNA structure, Gene and Genome Organization, chromosomes, Chromatin re-modelling, transposons
2


4


2
3
1
1
2

2
Replication, Recombina-tion and  Repair of DNA
2
1
2
4
2
4
3
2
3
4
3
3
2
2
Transcription and RNA processing
4
2
1
2
2
1
2
2
3
1
1
4
3
3
Translation, Translational regulation, post-translational  and Protein modifications
1
2
2
3
2
3
2
2
2
2
1
2
4
2
Gene regulation, operons, RNA interference, DNA methylation  etc.
4
2
2
3
2
2
3
3
2
3
3
3
3
1
Genomics & Application, Gene Therapy, Transgenic plants and animals, Mol markers and MAS
1
2
3
4
6
3
4
3
4
3
4
3
4
3
Viral molecular genetics

2
2
3
2
2
1





1
1

Total questions
14
11
12
19
20
15
15
14
17
14
13
17
17
14


  • Viral genetics, specifically the regulatory cascades are important. A thorough understanding of the lambda phage regulatory cascades may serve well, since it will clarify many concepts of prokaryotic gene regulation in general. Kindly go through with significance of CI/Cro system in viral life cycle. At grassroots, we devoted at least 4 hrs to this complicated topic of lambda phage. The lecture is supported by extensive illustrations to create a basic imagery of the entire process. We feel this task is well worth the effort in helping the students.

  • Finally, in applied biology section technologies for creating genetically modified plants and animals are important. Agrobacterium based gene transfer technology is a favorite topic. Site specific recombination, specifically with the Cre-Lox system, Molecular markers can be focused upon.

We hope this helps. An investment of time on this area of the syllabus is well worth the effort.


Grassroots  Academy
www.grassrootsacademy.in

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